Construction of Full-Length Goose Muscle cDNA Library
نویسندگان
چکیده
منابع مشابه
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Here, we describe a fast, simple method for constructing full-length cDNA libraries using SMART technology. This novel procedure uses the template-switching activity of Moloney murine leukemia virus (MMLV) reverse transcriptase to synthesize and anchor first-strand cDNA in one step. Following reverse transcription, three cycles of PCR are performed using a modified oligo(dT) primer and an ancho...
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ژورنال
عنوان ژورنال: Journal of Animal and Veterinary Advances
سال: 2012
ISSN: 1680-5593
DOI: 10.3923/javaa.2012.2106.2109